mircury microarray labeling kit Search Results


mircury microarray labeling kit  (Qiagen)
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Qiagen mircury microarray labeling kit
miR-19a is upregulated in the hypoxia-exposed CRC cells: (a) differentially expressed miRNAs between CRC cells cultured under hypoxic and normoxic conditions screened using a <t>microarray</t> analysis; (b) expression of miR-19a in HCT116 and SW480 cells cultured under hypoxic and normoxic conditions validated by RT-qPCR ( ∗ p < 0.05, two-way ANOVA); (c) expression of miR-19a in cancer and paracancerous tissues from CRC patients examined by RT-qPCR ( ∗ p < 0.05, paired t -test); (d) relevance of miR-19a expression to the survival of patients ( ∗ p < 0.05, Kaplan-Meier analysis); (e) relevance of miR-19a expression to the clinical characteristics of patients; (f) expression of miR-19a in HCT116 and SW480 and in normal NCM460 cells determined by RT-qPCR ( ∗ p < 0.05, one-way ANOVA). Data were presented as the mean ± SD from three independent experiments.
Mircury Microarray Labeling Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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miR-19a is upregulated in the hypoxia-exposed CRC cells: (a) differentially expressed miRNAs between CRC cells cultured under hypoxic and normoxic conditions screened using a microarray analysis; (b) expression of miR-19a in HCT116 and SW480 cells cultured under hypoxic and normoxic conditions validated by RT-qPCR ( ∗ p < 0.05, two-way ANOVA); (c) expression of miR-19a in cancer and paracancerous tissues from CRC patients examined by RT-qPCR ( ∗ p < 0.05, paired t -test); (d) relevance of miR-19a expression to the survival of patients ( ∗ p < 0.05, Kaplan-Meier analysis); (e) relevance of miR-19a expression to the clinical characteristics of patients; (f) expression of miR-19a in HCT116 and SW480 and in normal NCM460 cells determined by RT-qPCR ( ∗ p < 0.05, one-way ANOVA). Data were presented as the mean ± SD from three independent experiments.

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Hypoxia Enhances Activity and Malignant Behaviors of Colorectal Cancer Cells through the STAT3/MicroRNA-19a/PTEN/PI3K/AKT Axis

doi: 10.1155/2021/4132488

Figure Lengend Snippet: miR-19a is upregulated in the hypoxia-exposed CRC cells: (a) differentially expressed miRNAs between CRC cells cultured under hypoxic and normoxic conditions screened using a microarray analysis; (b) expression of miR-19a in HCT116 and SW480 cells cultured under hypoxic and normoxic conditions validated by RT-qPCR ( ∗ p < 0.05, two-way ANOVA); (c) expression of miR-19a in cancer and paracancerous tissues from CRC patients examined by RT-qPCR ( ∗ p < 0.05, paired t -test); (d) relevance of miR-19a expression to the survival of patients ( ∗ p < 0.05, Kaplan-Meier analysis); (e) relevance of miR-19a expression to the clinical characteristics of patients; (f) expression of miR-19a in HCT116 and SW480 and in normal NCM460 cells determined by RT-qPCR ( ∗ p < 0.05, one-way ANOVA). Data were presented as the mean ± SD from three independent experiments.

Article Snippet: The isolated miRNAs were labeled with Hy3 using a miRCURY microarray labeling kit (Exiqon) and then hybridized using a miRCURY LNA miRNA Array (v.8.0, Exiqon).

Techniques: Cell Culture, Microarray, Expressing, Quantitative RT-PCR